Glen Watt recently finished the first of two MRes projects at TAC. This was a challenging ‘high risk’ project and although we did not achieve everything we set out to Glen performed the work with a great deal of diligence and care. We are all very impressed with his work. Here is how it went in his own words…
“My first research project at TAC involved generating novel antibodies to a cell surface receptor expressed on multiple myeloma cells. I performed four hybridoma fusions in total. Successfully fused cells were grown to a suitable density before their supernatants were screened for the presence of antibody to cell surface receptor. Although antibody secreting hybridoma cells were successfully produced, unfortunately no cells positive for antibody to the cell surface receptor were identified. An alternative antibody generation method will be the used in my next project.
During the course of this project I learned valuable skills and techniques, including: culture of both adherent and suspension cells, hybridoma production, ELISA, limiting dilutions, antibody conjugations, molar calculations and dilutions. All of this was done whilst working in a ISO9001 quality accredited laboratory under the supervision of Eric Wagner.”
